*The picture in the header was taken from here.
This web page was produced as an assignment for Genetics 677, an undergraduate course at UW-Madison.
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Protein Homologs Information
Proteins that are from different organisms but are almost identical and have related functions are known as homologs. Homologous proteins are usually inherited from the same common ancestor. Studies on homologs are useful as it allows us to determine how similar 2 organisms are. Besides that, if a protein is well conserved across many species, it is most likely that it plays an important role in the fitness or survival of the organisms.
Below is the list of organisms that possess the homologous UBE3A protein:
Organism: Pan troglodytes (chimpanzee)
Protein Name: ubiquitin-protein ligase E3A isoform 12 Accession Number: XP_001161311.1 E value: 0.0 Identities: 99% FASTA (NCBI): Here FASTA (.txt): Here Organism: Mus muculus (mouse) Protein Name: ubiquitin-protein ligase E3A isoform 2 Accession Number: NP_035798.2 E value: 0.0 Identities: 95% FASTA (NCBI): Here FASTA (.txt): Here Organism: Gallus gallus (chicken) Protein Name: ubiquitin-protein ligase E3A Accession Number: XP_416882.1 E value: 0.0 Identities: 94% FASTA (NCBI): Here FASTA (.txt): Here |
Organism: Bos taurus (cattle)
Protein Name: ubiquitin-protein ligase E3A Accession Number: NP_001091932.1 E value: 0.0 Identities: 97% FASTA (NCBI): Here FASTA (.txt): Here Organism: Rattus norvegicus (rat) Protein Name: ubiquitin-protein ligase E3A Accession Number: NP_001178766.1 E value: 0.0 Identities: 96% FASTA (NCBI): Here FASTA (.txt): Here Organism: Danio rerio (zebrafish) Protein Name: ubiquitin-protein ligase E3A Accession Number: NP_001007319.1 E value: 0.0 Identities: 86% FASTA (NCBI): Here FASTA (.txt): Here |
Analysis
Homologs of Ubiquitin-protein ligase E3A Isoform II were determined by using homologene. Homologene returned 9 homologs but the predicted sequence for Canis lupus (gray wolf) is obsolete and there is not an updated version. blastp was later used to find homologs in C. lupus but there are not any significant similarities. Homologene also showed homologs in Drosophila melanogaster (fruit fly) and Anopheles gambiae (mosquito) but when comparing these protein sequences with the human copy via blastp, the identities were less than 50%. Therefore, homologs in C. lupus, D. melanogaster and A. gambiae were discarded.
Besides the 6 listed organisms, search for homologs of Ubiquitin-protein ligase E3A Isoform II in other organisms, such as Macaca mulatta (rhesus monkey), Cavia porcellus (guinea pig), and Arabidopsis thaliana (a plant), were performed too via blastp. Unfortunately, blastp did not return any homologs with good E value or similarities (>60%).
The list of homologs shows that the Ubiquitin-protein ligase E3A Isoform II protein is highly conserved in mammals, with 4 mammals (chimpanzee, cattle, mouse and rat) having the homolog. On the contrary, there is one homolog in the birds class (chicken) and fish class (zebrafish) respectively.
Homologs of Ubiquitin-protein ligase E3A Isoform II were determined by using homologene. Homologene returned 9 homologs but the predicted sequence for Canis lupus (gray wolf) is obsolete and there is not an updated version. blastp was later used to find homologs in C. lupus but there are not any significant similarities. Homologene also showed homologs in Drosophila melanogaster (fruit fly) and Anopheles gambiae (mosquito) but when comparing these protein sequences with the human copy via blastp, the identities were less than 50%. Therefore, homologs in C. lupus, D. melanogaster and A. gambiae were discarded.
Besides the 6 listed organisms, search for homologs of Ubiquitin-protein ligase E3A Isoform II in other organisms, such as Macaca mulatta (rhesus monkey), Cavia porcellus (guinea pig), and Arabidopsis thaliana (a plant), were performed too via blastp. Unfortunately, blastp did not return any homologs with good E value or similarities (>60%).
The list of homologs shows that the Ubiquitin-protein ligase E3A Isoform II protein is highly conserved in mammals, with 4 mammals (chimpanzee, cattle, mouse and rat) having the homolog. On the contrary, there is one homolog in the birds class (chicken) and fish class (zebrafish) respectively.
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Created by Jonathan Mok
[email protected]
Last updated 02/23/2012
Genetics 677
Created by Jonathan Mok
[email protected]
Last updated 02/23/2012
Genetics 677